Horizontal Electrophoresis

Amersham Biosciences

This systems performs electrophoresis with submerged horizontal gels that offer many advantages for nucleic acid and protein separations and a primary tool for molecular biology research. Four different systems are available; Mini Submarine Electrophoresis Unit, Submarine Electrophoresis Unit, GenePhor Electrophoresis Unit and MultiPhor II Electrophoresis System.

Mini Submarine Electrophoresis Unit

Hoefer HE 33

This horizontal agarose unit is intended for rapid electrophoresis of small quantities of nucleic acids in agarose gels. The base of the unit holds coolant that can be chilled before the run. This passive cooling capacity allows fast, high voltage runs.

Specifications

• Max. sample well number: 8
• Max. voltage: 500 V for 5 minutes or less
• Max. wattage: 15 W
• Max. Current: 500 mA
• Coolant required: ~600 ml 50/50 water/ethylene glycol
• Gel size: 7 x 10 cm
• Size of the nucleotide to be separated: to 23 kb pairs

Submarine Electrophoresis Unit

Hoefer HE 99X

This unit electrophoretically separates nucleic acid fragments in a submarine gel. Gels can be casted in three different lengths.

Specifications

• Max. sample well number: 15
• Max. voltage: 200V
• Max. wattage: 20 W
• Max. Current: 100 mA
• Gel size: 15 cm wide x 10, 15 or 20 cm long
• Size of the nucleotide to be separated: to 23 kb pairs

GenePhor Electrophoresis Unit

GenePhor Electrophoresis Unit is a compact, temperature controlled, flatbed electrophoresis unit that requires no liquid running buffer and provides clean, efficient separation of DNA fragments for detection by isotopic or non-isotopic methods. With the touch of a button, the integrated solid-state Peltier cooling system can achieve any temperature between 5 and 65°C within minutes, reducing lengthy equilibration time. More importantly, the cooling system and safety lid assure even, stable temperature over the entire gel surface throughout the run, a critical factor for consistent separations using temperature-sensitive techniques.

Applications

It is designed for DNA applications such as electrophoretic analysis of,

• PCR fragments,
  restriction fragment length polymorphism (RFLP),
• heteroduplex analysis, single strand conformation polymorphism analysis (SSCP),
• random amplified polymorphic DNA (RAPD) and
• variable number of tandem repeats (VNTR).

Specifications

• Max. voltage: 1000 V
• Power consumption max.: 100 W
• Programming temperature range: 5.0-65.0°C
• Dimensions (WxDxH): 2380 x 350 x 150 mm

Multiphor II Electrophoresis Systems

This electrophoresis system is a versatile modular system for horizontal electrophoresis for both Nucleotide fragment and protein analysis. The modular design of Multiphor? II gives it the flexibility to handle virtually any flatbed electrophoretic technique. It is particularly well-suited for ultra-thin gels (0.1-0.5-mm) on glass or plastic supports up to 20 × 26 cm. In addition to accommodating gels of different sizes, the electrodes make secure, uniform contact with various types of buffer strips, eliminating the need for large volumes of liquid buffers.

Applications

• Configure the system for 1-D or 2-D protein electrophoresis, isoelectric focusing, or DNA fragment analysis.
• Select from a wide range of precast gels or cast your own gels for unique applications.
• Allows cooling of ultra-thin gels efficiently and uniformly, through the ceramic cooling plate, for improved resolution and speed at high voltages.
• To be used with adjustable electrodes allowing the use of different sized gels.

Specifications

• Max. Voltage: 3500 Vp-p(+/- 1750 V with reference to Ground)
• Max. power: 100 W
• Max. pressure cooling plate: 0.5 bar
• Dimensions: 16 x 31 x 40 cm
• Size of the nucleotide to be separated: to 23 kb pairs